FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200672

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200672
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8681764
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC93020.10714412416647123No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG86970.10017549428894865No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT466000.073.050011
AGCAGTG527400.064.475972
CAGTGGT670050.050.5550774
GCAGTGG703700.048.3545723
AGTGGTA706900.047.9574135
TGGTATC720650.046.970897
GTGGTAT723150.046.8306586
GGTATCA732250.046.251618
GTATCAA749100.045.2587139
CAACGCA729700.026.86249212-13
ATCAACG731050.026.83606110-11
AGAGTAC760500.025.41149718-19
ACGCAGA774400.025.35063714-15
AGTACAT761150.025.1438820-21
TACATGG754200.025.11766622-23
GCAGAGT782200.024.97031416-17
CATGGGA329750.023.56878324-25
TCAACGC725200.023.41942612-13
CGCAGAG741200.022.91714516-17
TATCAAC752800.022.62847310-11
Produced by FastQC (version 0.11.5)