FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200678

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200678
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8766085
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC93220.10634165650914862No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT465550.072.228941
AGCAGTG520600.064.434622
CAGTGGT670650.049.8916474
GCAGTGG698100.048.1039123
AGTGGTA700250.047.7859735
TGGTATC713300.046.8356067
GTGGTAT719150.046.4363566
GGTATCA730650.045.776588
GTATCAA746550.044.9038479
CAACGCA724350.026.79927312-13
ATCAACG727950.026.64531710-11
AGAGTAC755250.025.38372818-19
ACGCAGA769250.025.25502214-15
AGTACAT756550.025.09896720-21
GCAGAGT775300.024.99886116-17
TACATGG751750.024.95279322-23
TCAACGC720450.023.3163712-13
CGCAGAG736500.022.86188716-17
CATGGGA327100.022.84576424-25
CATGGGG299750.022.54076824-25
Produced by FastQC (version 0.11.5)