FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200682

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200682
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8543044
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC92110.10781871192516392No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT454950.073.1761251
AGCAGTG514050.064.57692
CAGTGGT659800.050.337074
GCAGTGG689100.048.3290333
AGTGGTA691800.047.9863555
TGGTATC705950.046.9221847
GTGGTAT712700.046.519256
GGTATCA718700.046.115048
GTATCAA736500.045.0466779
ACGACGC3700.033.3067555
CGACGCT4500.027.3853956
CAACGCA715950.026.90469612-13
ATCAACG717150.026.8645810-11
AGAGTAC749550.025.48871418-19
ACGCAGA761100.025.39948514-15
TACATGG742250.025.20811522-23
AGTACAT750400.025.19690920-21
GCAGAGT768650.025.09282916-17
CACGACG4950.024.894794
TCAACGC711850.023.35518612-13
Produced by FastQC (version 0.11.5)