FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200686

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200686
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8557634
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC93370.10910726025441145No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT452050.072.272571
AGCAGTG512550.063.6572382
CAGTGGT655800.049.7636644
GCAGTGG685650.047.774683
AGTGGTA689850.047.3656165
TGGTATC708200.046.0551387
GTGGTAT710500.045.9294366
GGTATCA719100.045.4069758
GTATCAA734250.044.491419
CAACGCA715500.026.70265812-13
ATCAACG716450.026.70174810-11
AGAGTAC747550.025.26965318-19
ACGCAGA760000.025.17108214-15
TACATGG739100.025.01259622-23
AGTACAT747250.025.0085420-21
GCAGAGT764800.024.91380316-17
CATGGGA320400.023.24424624-25
TCAACGC711500.023.02093512-13
CGCAGAG727200.022.55777716-17
CATGGGG290300.022.22058524-25
Produced by FastQC (version 0.11.5)