FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200688

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200688
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9810815
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC103080.10506772373141274No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG98450.10034844199997657No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT505350.072.346071
AGCAGTG574200.063.5096662
CAGTGGT729500.049.878834
GCAGTGG767500.047.636873
AGTGGTA767750.047.4729845
TGGTATC783800.046.3432357
GTGGTAT785650.046.2978256
GGTATCA796000.045.735778
GTATCAA817350.044.6109629
CAACGCA794200.026.7140612-13
ATCAACG796650.026.65208410-11
AGAGTAC830800.025.26719518-19
ACGCAGA844550.025.15478314-15
AGTACAT830500.025.06460220-21
TACATGG824450.024.971722-23
GCAGAGT848600.024.92037616-17
TCAACGC789000.023.15216612-13
CATGGGA356650.022.93433624-25
CATGGGG327050.022.87225724-25
CGCAGAG805600.022.6658816-17
Produced by FastQC (version 0.11.5)