FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200694

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200694
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9899290
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC106690.107775406114984No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT515750.072.660461
AGCAGTG581450.064.4396062
CAGTGGT749800.049.9551124
GCAGTGG780750.048.087183
AGTGGTA788350.047.5515065
TGGTATC805250.046.4319087
GTGGTAT809350.046.185966
GGTATCA818850.045.7147148
GTATCAA835850.044.876249
ACGACGC3350.027.9089415
CAACGCA813950.026.67108312-13
ATCAACG815050.026.62652610-11
AGAGTAC848650.025.18336718-19
ACGCAGA865650.025.1325714-15
TACATGG836100.025.06473222-23
AGTACAT850900.024.90006420-21
GCAGAGT869250.024.8065216-17
TCAACGC808600.023.1709912-13
CATGGGA372350.023.16172624-25
CGCAGAG828150.022.66328816-17
Produced by FastQC (version 0.11.5)