FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200696

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200696
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11329516
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC121620.1073479220118494No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT592450.072.689161
AGCAGTG669400.064.276432
CAGTGGT856500.050.1576964
GCAGTGG896150.048.0712853
AGTGGTA904850.047.587025
TGGTATC924000.046.449787
GTGGTAT928850.046.2292986
GGTATCA940300.045.6774258
GTATCAA959450.044.7671179
ACGACGC3950.029.0407645
CAACGCA931950.026.75000412-13
ATCAACG935100.026.68836810-11
AGAGTAC973250.025.32411218-19
ACGCAGA991050.025.18785314-15
TACATGG959050.025.1636222-23
AGTACAT973650.025.08108120-21
CGACGCT4600.024.9396026
GCAGAGT996950.024.93725816-17
CATGGGA416900.023.43356524-25
TCAACGC926600.023.19428412-13
Produced by FastQC (version 0.11.5)