FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200700

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200700
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11429924
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC123940.10843466675718928No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT604500.072.523571
AGCAGTG685650.063.8342972
CAGTGGT868300.050.415144
GCAGTGG905250.048.47023
AGTGGTA917800.047.717535
TGGTATC932450.046.7937977
GTGGTAT936500.046.6726956
GGTATCA951150.045.95978
GTATCAA969400.045.1042149
CAACGCA944000.026.88772212-13
ATCAACG946750.026.81322910-11
ACGACGC3650.025.610825
AGAGTAC989600.025.35711918-19
ACGCAGA1008450.025.21590414-15
AGTACAT992100.025.09930220-21
TACATGG980400.025.062322-23
GCAGAGT1014400.024.9595116-17
TCAACGC938800.023.3585812-13
CATGGGA420800.023.28714824-25
CGCAGAG960650.022.88318816-17
Produced by FastQC (version 0.11.5)