FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200704

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200704
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8926793
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN158690.17776820858285836No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC96380.10796710531990603No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG89890.10069685720280508No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT479150.072.3821951
AGCAGTG537300.064.45472
CAGTGGT685100.050.480564
GCAGTGG712150.048.716293
AGTGGTA718800.048.123545
TGGTATC733300.047.0916867
GTGGTAT738250.046.7670256
GGTATCA745600.046.323918
GTATCAA761000.045.3545079
CAACGCA736550.027.0794212-13
ATCAACG739550.026.9853710-11
AGAGTAC766800.025.67575618-19
ACGCAGA787100.025.41098214-15
AGTACAT769450.025.39167420-21
TACATGG765350.025.27225722-23
GCAGAGT788900.025.21450616-17
TCAACGC734750.023.53384412-13
CATGGGA332050.023.32184624-25
ACGACGC3300.023.1707325
CGCAGAG749750.023.11023716-17
Produced by FastQC (version 0.11.5)