FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200716

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200716
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8803233
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN109560.1244542771956621No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC94770.10765363134203082No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG93100.10575660101237808No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT470600.072.7603151
AGCAGTG528900.064.65892
CAGTGGT672500.050.845924
GCAGTGG705350.048.576973
AGTGGTA707450.048.341095
TGGTATC723100.047.175497
GTGGTAT728000.046.8966836
GGTATCA737000.046.3290148
GTATCAA751750.045.4721689
CAACGCA733550.026.93214812-13
ATCAACG734200.026.89542210-11
AGAGTAC763400.025.49601618-19
ACGCAGA781500.025.31768414-15
AGTACAT765650.025.20481320-21
TACATGG757200.025.1605322-23
GCAGAGT782200.025.1599816-17
TCAACGC728500.023.5193312-13
CATGGGA328150.023.1845924-25
CGCAGAG744850.023.02654516-17
TATCAAC757900.022.6675710-11
Produced by FastQC (version 0.11.5)