FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200718

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200718
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8714537
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN147060.16875251089071053No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC93650.10746411427250813No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT462400.072.85261
AGCAGTG521900.064.430392
CAGTGGT670000.050.0898134
GCAGTGG699000.048.22613
AGTGGTA705800.047.658875
TGGTATC717550.046.716937
GTGGTAT720950.046.5421646
GGTATCA728950.046.012038
GTATCAA745900.045.018249
CAACGCA727150.026.699112-13
ATCAACG729300.026.6343510-11
ACGACGC3050.026.4711825
AGAGTAC756500.025.25422918-19
ACGCAGA773100.025.1789114-15
AGTACAT755650.025.07466920-21
TACATGG749000.025.01116222-23
GCAGAGT773800.024.9091816-17
TCAACGC722000.023.30172312-13
CATGGGA326900.023.18287324-25
CGCAGAG737650.022.84277716-17
Produced by FastQC (version 0.11.5)