FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200720

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200720
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10084782
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN182640.1811045593251297No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC108580.1076671761471889No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG102650.10178702920896059No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT526050.072.8569261
AGCAGTG590300.064.748792
CAGTGGT757200.050.4411934
GCAGTGG797600.048.032033
AGTGGTA796750.047.9244355
TGGTATC809250.046.995327
GTGGTAT813850.046.7582246
GGTATCA825200.046.130228
GTATCAA839850.045.403469
ACGACGC3550.029.9176675
CAACGCA819650.026.95459212-13
ATCAACG820250.026.92603310-11
AGAGTAC855100.025.53652618-19
ACGCAGA874300.025.3359214-15
TACATGG847900.025.23052422-23
AGTACAT857500.025.2276620-21
GCAGAGT877100.025.1161616-17
CATGGGA369900.023.49773824-25
TCAACGC814850.023.42889612-13
CGCAGAG832450.023.0146816-17
Produced by FastQC (version 0.11.5)