FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200722

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200722
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10072680
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN209680.208167041939186No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC105630.10486782067930282No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT532700.072.849481
AGCAGTG603150.064.187082
CAGTGGT765900.050.4475174
GCAGTGG806550.048.005783
AGTGGTA805500.048.0026475
TGGTATC822500.046.8681537
GTGGTAT826900.046.6662036
GGTATCA836750.046.1286058
GTATCAA855850.045.1596459
ACGACGC3200.033.1955155
ATCAACG833700.026.8884510-11
CAACGCA834700.026.88390712-13
AGAGTAC869950.025.48455418-19
ACGCAGA886100.025.38453514-15
TACATGG858450.025.31480222-23
AGTACAT871700.025.22506120-21
GCAGAGT893200.025.04714616-17
CACGACG4450.023.8691484
CATGGGA370250.023.469924-25
TCAACGC828100.023.4029712-13
Produced by FastQC (version 0.11.5)