FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200726

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200726
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10063973
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN170700.1696149224565686No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC107330.10664774239756009No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT522200.072.477821
AGCAGTG591450.063.832262
CAGTGGT752250.050.1439744
GCAGTGG787900.047.9306223
AGTGGTA790250.047.7615365
TGGTATC814600.046.2540747
GTGGTAT821500.045.874096
GGTATCA831800.045.350538
GTATCAA849300.044.4963579
CAACGCA825450.026.67858112-13
ATCAACG828250.026.61608510-11
AGAGTAC860500.025.19234318-19
ACGCAGA875350.025.17306914-15
TACATGG851550.025.0232722-23
AGTACAT861900.024.96478520-21
GCAGAGT879400.024.86350416-17
ACGACGC3900.023.9712185
TCAACGC819950.023.10997212-13
CATGGGA378150.023.07509224-25
CGCAGAG834850.022.6622816-17
Produced by FastQC (version 0.11.5)