FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200728

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200728
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11647205
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN206550.17733868340086742No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC126280.10842086148565257No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT617350.072.088091
AGCAGTG689650.064.394082
CAGTGGT884400.050.1062134
GCAGTGG922900.048.1971663
AGTGGTA927550.047.83355
TGGTATC947200.046.745077
GTGGTAT951850.046.5270966
GGTATCA963750.046.0553368
GTATCAA985450.045.0823149
ACGACGC3850.029.7924145
CAACGCA957450.026.81986812-13
ATCAACG961250.026.7125210-11
AGAGTAC1001950.025.27036318-19
ACGCAGA1021500.025.1713314-15
AGTACAT1000850.025.116320-21
TACATGG992300.025.03690322-23
GCAGAGT1024350.024.96835116-17
CGACGCT4850.023.6516866
TCAACGC953050.023.38984112-13
CATGGGA429150.023.18011324-25
Produced by FastQC (version 0.11.5)