FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200730

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200730
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11629891
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN240090.2064421755973465No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC124100.10670779287613272No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT616400.072.5015951
AGCAGTG692150.064.336172
CAGTGGT884600.050.2813224
GCAGTGG921700.048.350263
AGTGGTA931400.047.802115
TGGTATC947500.046.886877
GTGGTAT949950.046.7684026
GGTATCA966650.046.029748
GTATCAA989450.045.055569
ACGACGC4350.027.3510285
CAACGCA957250.026.77576612-13
ATCAACG960750.026.70418710-11
AGAGTAC1000800.025.25151618-19
ACGCAGA1019700.025.15619914-15
AGTACAT996700.025.0771620-21
TACATGG988050.025.01939822-23
GCAGAGT1021950.024.93883316-17
CGACGCT4700.023.5074086
TCAACGC950950.023.43597212-13
CATGGGA429450.023.26391424-25
Produced by FastQC (version 0.11.5)