FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200732

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200732
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11727805
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN145730.12426025159865807No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC124060.10578279567233595No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT611700.072.4027561
AGCAGTG688150.064.242672
CAGTGGT885600.049.9085044
GCAGTGG927850.047.6549263
AGTGGTA932450.047.4170045
TGGTATC952300.046.3253147
GTGGTAT958800.046.0397266
GGTATCA971200.045.4782528
GTATCAA993050.044.5563359
CAACGCA960400.026.73361412-13
ATCAACG965350.026.60250910-11
AGAGTAC1004700.025.24975218-19
ACGCAGA1023750.025.1084414-15
AGTACAT1004550.025.02998720-21
GCAGAGT1024150.024.95260416-17
TACATGG996500.024.8869822-23
ACGACGC3850.024.2793925
CATGGGA431250.023.21965624-25
TCAACGC954950.023.150212-13
CGCAGAG975400.022.711816-17
Produced by FastQC (version 0.11.5)