FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200734

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200734
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11606139
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN196660.16944480847592813No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC124240.10704679652725164No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT610450.072.683991
AGCAGTG691050.064.129372
CAGTGGT880650.050.2422754
GCAGTGG920100.048.214073
AGTGGTA930850.047.6393855
TGGTATC952900.046.4340067
GTGGTAT957000.046.2601176
GGTATCA968400.045.692778
GTATCAA989950.044.7457059
CAACGCA959900.026.80742612-13
ATCAACG964950.026.6947810-11
AGAGTAC1005350.025.30452518-19
ACGCAGA1022000.025.24128214-15
AGTACAT1004300.025.17076120-21
TACATGG995100.025.12989422-23
GCAGAGT1027050.024.97074916-17
TCAACGC955550.023.20884312-13
CATGGGA430300.022.89147424-25
CGCAGAG976800.022.73301116-17
TATCAAC993200.022.39027410-11
Produced by FastQC (version 0.11.5)