FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200736

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200736
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46842811
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG591680.12631180481461712No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG28050.017.50911726
AGCTTAG216050.013.79783745
GGAGGTC310150.013.7129294
GGGAGGT323150.013.6427443
GGCATAG227600.012.86948335
ATAGGGG233250.012.83299138
GAGGTCA345950.012.5842685
GTCACTC343350.012.4149578
GGTCACT344550.012.1872067
ACTCTCC355250.011.89970211
TGGCATA250050.011.85574734
GAGCTTA253400.011.79202644
ATATAGC47450.011.5490926
TGTCGTT44350.011.42995328
AGGTCAC387950.011.2021016
TCACTCT386000.011.1248449
TGGGAGG293050.011.0857632
CACTCTC390600.010.83853410
CATAGGG287350.010.50966837
GCATAGG301000.09.99335236
Produced by FastQC (version 0.11.5)