FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200740

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200740
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49033512
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG96300.011.93629745
ATAGGGG102050.011.79435838
ACCCGGC69950.011.350585
GGCATAG100400.011.27173935
CATAGGG108150.010.52646137
GGGAGGT196950.010.2083353
ATAGCTC68700.010.11858439
GTCACTC169950.010.1039218
GAGCTTA116000.010.06417444
GCATAGG115950.09.85714936
GGTCACT174900.09.5984877
TCACCCG85550.09.5424533
ACTCTCC184300.09.31749411
GTTGTCG13850.09.26500726
TCAGACG74200.09.2132892
CGTAGGG57350.09.101537
TAATCGG33000.08.9284124
TAGCTCT79250.08.85591440
GCGTAGG59050.08.83947536
GGCGTAG59850.08.60850935
Produced by FastQC (version 0.11.5)