FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200743

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200743
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45962565
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT465430.10126284292445384No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC135400.017.584894
CAGACCG149650.016.3615633
CGTTACG21450.016.05544521
GACCGCG147400.015.970075
GAACGAT149600.015.39806645
ACCGCGT156400.015.2237486
CCGTACG35200.015.1522924
GTCCGTT35450.014.7308111
CCGCGTT163750.014.5129357
TTCGACG13400.014.44329556
CGCGTTC165500.014.3594758
CGTACGC37350.014.2198185
CCGGCTT178100.013.4769962
AACGCTA154000.013.35961923
GCCGGCT154700.013.2405481
TCAGACC194200.013.1705482
ACGCTAC156750.013.06779924
CCCGGAT108250.013.05831
TTCACGC172000.012.9663537
TCACGCT173600.012.743148
Produced by FastQC (version 0.11.5)