FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200747

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200747
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54835602
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1030640.18795088636028834No Hit
GCCGGCTTCACGCTCAGGAGAAAAACGCTACCTCTCTTCCTCGTGGTTTTC914030.16668550479303573No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG766600.1397996870719136No Hit
CTCAGGAGAAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACAC720010.13130338206189474No Hit
CCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGA597090.10888728822563123No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG29350.022.9986676
AGACCGC239700.022.3220944
GACCGCG236350.022.3147245
GCCGGCT410700.021.8410051
CAGACCG247300.021.7179783
ACCGCGT246150.021.572566
CCGGCTT506700.020.1646962
TCAGACC297800.018.8133322
CCGCGTT282150.018.8047127
TTCACGC547700.018.4872687
CGCGTTC291000.018.2869768
CGGTATA46150.018.28211620
CGTTACG49800.018.11693621
TCACGCT559250.018.105498
CGGCTTC579000.018.0042423
GTTCGAC37000.018.000295
CTCAGAC351400.017.8354241
TCGACGA37550.017.797117
TCGTTAC29950.016.90313744
CCCGTAT41000.016.5761281
Produced by FastQC (version 0.11.5)