FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200748

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200748
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54835602
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA1200850.21899093949948795No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG1061940.19365885688644396No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG758200.138267835556907No Hit
ATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGA572840.10446497879242758No Hit
CAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAG561820.10245533549535937No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT346900.015.7854333
GGAGGTC348300.015.3860984
GTTGTCG37850.014.5627326
GGTCACT389350.013.4644437
GTCACTC393800.013.0210158
ATAGGGG327950.012.98239238
GGCATAG330350.012.71017835
AGGGATC262800.012.6610682
AGCTTAG339250.012.5277945
TGGGAGG367950.012.22837452
TGGCATA353500.011.93508134
CTGGGAG407450.011.8152791
GAGCTTA357900.011.77542344
ACTCTCC439850.011.76613911
ATAATCG84850.011.66651253
TAATCGG82050.011.4888264
GAGGTCA480350.011.46589855
CAGGGAT312300.011.3740391
GTAACGA79200.011.33511518
GCGTCGT37300.011.28089411
Produced by FastQC (version 0.11.5)