FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200749

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200749
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47894424
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT896060.18709067260105267No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC202650.020.9963134
CAGACCG206450.020.7515343
GACCGCG216300.019.7025135
ACCGCGT221700.019.2228726
GAACGAT224350.019.09918445
CCGCGTT236350.018.0587867
CGCGTTC238350.017.9442258
TTCGGCG74600.017.9143644
CCGTACG77050.017.1712884
CGTACGC80550.016.5369075
GCGTTCT260200.016.4719689
TCAGACC274200.016.0919342
AGAACGA266700.016.06192844
GTCCGTT40850.015.818031
CTCAGAC304600.015.7660741
AGACGCT198000.015.22626341
TACGGAG288600.014.89759834
TACGCCA89750.014.891177
AATACGG289050.014.87446732
ATACGGA289550.014.85650533
Produced by FastQC (version 0.11.5)