FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200752

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200752
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50197726
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGTA18050.011.34184629
CGCGCGT18100.011.06193328
AGCTTAG90950.010.95649545
TTAGCCC63550.010.87420752
GGCATAG99800.010.25724535
ATAACGC55150.010.07743239
TAACGCC56300.09.91155240
GGGAGGT156250.09.8672943
ATAGGGG108600.09.73789738
TCAGACG75750.09.3902952
CATAGGG109050.09.36712937
CTTAGCC82000.09.280231
CGTTAAT36750.09.2507181
GGGCGTA26800.09.15095218
GTTGTCG18500.09.12004526
GCATAGG107400.09.11283536
TAATCGG68550.08.9962154
GGAGGTC175150.08.7123694
GCGCGCG23800.08.69603627
GAGCTTA116450.08.61693244
Produced by FastQC (version 0.11.5)