FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200753

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200753
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44117402
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1305720.2959648439860534No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA624980.1416629202236342No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG602060.13646769136586964No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT276350.023.2020445
CGTTACG20150.018.3170621
TTCGACG17400.018.2309446
AGAACGA353350.018.17165844
AGACCGC374550.017.1849124
CAGACCG380900.016.9514273
GACCGCG405750.015.8024935
ACCGCGT412950.015.5760016
CGCGTTC417100.015.4533888
CCGCGTT418450.015.3981567
CTCAGAC451850.015.1726771
TCAGACC433550.015.1108042
GCGTTCT431350.015.0054629
TCGACGA21800.014.9641077
TTCGGCG121400.014.80722844
AATACGG435900.014.70588432
ATACGGA439700.014.594110533
AAGAACG441650.014.53856143
TACGGAG441150.014.50509234
TCGGCGC121900.014.02654345
Produced by FastQC (version 0.11.5)