FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200755

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200755
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46957238
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG679240.14465075650318274No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT581930.12392764668143386No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG27350.021.5505316
AGACCGC155200.020.0177844
ACCGCGT157750.019.6228966
GACCGCG157700.019.5720585
GCCGGCT297000.019.5655631
CAGACCG164300.019.1555293
CCGGCTT345400.019.1161212
TTCACGC359450.017.711777
TCACGCT360200.017.6998738
CGGCTTC386750.017.3922163
CCGCGTT182200.017.0513737
CGTTACG17850.016.89460621
ACGCTCA403750.016.02471710
GTTCGAC35850.015.9389085
CGCGTTC157250.015.7224818
CACGCTC409450.015.7137269
TCGACGA38600.015.6192997
CCGTACG61700.015.6053194
CGTACGC63050.015.4495875
CGGTATA54200.015.44288520
Produced by FastQC (version 0.11.5)