FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200757

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200757
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47310476
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT580940.12279309977773209No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT152350.018.96116845
AGACCGC151700.018.775564
ACCGCGT152550.018.523276
GACCGCG158900.017.783195
TTCGACG21150.017.7642296
CAGACCG163000.017.7223873
CGTTACG19100.017.43545721
CCGCGTT163950.017.2764537
CGCGTTC164550.017.240828
GTCCGTT39650.016.7463861
AGAACGA190600.015.20313744
TCAGACC201750.014.7087612
ATACGGA202450.014.25942133
AATACGG201450.014.25286532
TACGGAG200250.014.24764134
AACGCTA110650.014.11307823
ACGCTAC110950.014.09533224
AAGAACG208700.013.89538943
GCGTTCT206250.013.8750279
CCCGTAT43050.013.8029841
Produced by FastQC (version 0.11.5)