FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200760

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200760
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49484372
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG601200.12149290285021702No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG195900.014.97518145
ATAGGGG208400.014.306334538
GTTGTCG26950.013.60576726
GGCATAG214550.013.56917835
GGGAGGT314950.013.2407383
GTCACTC316550.012.7177768
TGGCATA231450.012.71447334
GGTCACT318050.012.6144117
GAGCTTA236350.012.43337444
GGAGGTC334250.012.3746044
GAGGTCA341100.012.2177815
CATAGGG247050.012.04930237
ACTCTCC336450.011.99897611
AGGTCAC355100.011.8559766
GCATAGG263800.011.3261936
CACTCTC370000.010.92312510
TCACTCT383550.010.7425059
TGGGAGG305100.010.4598512
TAGGGGA395300.010.18347339
GCTCTGT404600.010.07035422
Produced by FastQC (version 0.11.5)