FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200761

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200761
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50402747
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1335850.2650351577067813No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC289250.022.9113394
GAACGAT295400.022.1692445
CAGACCG301100.022.1291833
GACCGCG314600.020.9436195
ACCGCGT334300.019.7430366
CCGCGTT346000.019.0484737
CGCGTTC352550.018.6870638
AGAACGA351950.018.65346744
CTCAGAC392350.018.4133871
TCAGACC369500.018.3790992
GCGTTCT368100.017.8989099
ATACGGA381500.017.1518533
AATACGG383750.017.10398932
TACGGAG384250.017.0411634
CGTACGC89000.016.7071555
TTCGGCG87200.016.64173144
CCGTACG89300.016.6258374
AAGAACG395150.016.62093443
CAATACG397300.016.59945531
CGTTACG19800.016.13080621
Produced by FastQC (version 0.11.5)