FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200762

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200762
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50402747
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG826860.16405058240178855No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA566370.11236887545037971No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG255400.015.93287545
GGGAGGT378850.015.5322923
GGTCACT376150.015.1075377
GTCACTC385400.014.8564538
ATAGGGG284950.014.49842538
AGGTCAC404750.014.3713036
GGCATAG285400.014.30176835
GGAGGTC413750.014.1158444
ACTCTCC413550.013.97830711
GAGGTCA425200.013.80460555
GAGCTTA296950.013.74150544
TCACTCT422050.013.6692189
CACTCTC429450.013.32487210
TGGGAGG330200.013.1201452
TGGCATA318000.012.89210334
CATAGGG345500.012.02271637
GTTGTCG21550.011.90797826
CTGGGAG420250.011.541941
GCATAGG362150.011.53210136
GCTCTGT509000.011.35074922
Produced by FastQC (version 0.11.5)