FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200765

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200765
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52433900
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1310780.24998712664898093No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT236700.027.11523645
AGACCGC281350.022.910114
AGAACGA287350.022.30441944
CAGACCG290900.022.289663
GACCGCG309400.020.8039675
ACCGCGT315800.020.4037276
CCGCGTT321600.020.035757
CGCGTTC323500.019.9458928
TACGGAG326450.019.56750534
ATACGGA328050.019.52697633
AAGAACG330900.019.41637443
AATACGG331500.019.34411432
GCGTTCT337750.019.1909549
CAATACG346100.018.61911231
CTCAGAC379750.018.4331041
TCAGACC360150.018.253752
ACGGAGA356250.018.04970235
CCCAATA373750.017.34401529
CGGAGAG371150.017.21020936
CCGTACG53100.016.3123174
Produced by FastQC (version 0.11.5)