FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200766

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200766
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52433900
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG110200.012.1479538
GTTGTCG19100.011.66057926
AGCTTAG118750.011.30891245
GGCATAG120650.010.87072935
GGGAGGT193950.010.7578223
CATAGGG132400.010.127537
GAGCTTA142400.09.62186244
GCATAGG142300.09.5174536
ACTCTCC224050.08.97946811
GTCACTC230200.08.9060868
GGTCACT224850.08.8477567
TGGCATA153900.08.8282134
GGCGTAG65900.08.77334435
GGAGGTC242750.08.7329824
GCGTAGG65750.08.69094536
CGTAGGG66150.08.53665737
TAATCGG31050.08.0451154
GCGTTCG19450.07.982608315
CACTCTC261150.07.858912510
CCGCGTT21300.07.817902613
Produced by FastQC (version 0.11.5)