FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200770

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200770
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46659114
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG20100.012.42586426
ATAGGGG103200.010.68277238
GGCATAG110050.09.69074635
ACACTCG89450.09.6599584
AGCTTAG119200.09.53169445
CACTCGA90500.09.1997215
CGTAACG9850.09.13648742
TAATCGG68900.09.0792494
GTAACGA68100.08.98688918
AACTCTA84900.08.8775345
GTCACTC153950.08.8420048
CATAGGG124600.08.649437
ATAATCG75350.08.600763
GGTCACT156650.08.5890637
AATCGGT75650.08.5367885
GGGAGGT182500.08.4830723
GCACCGA182550.08.45467444
TCTATAG190350.08.3814853
GCATAGG127850.08.376760536
ATCGGTT77700.08.3683486
Produced by FastQC (version 0.11.5)