FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200772

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200772
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51532347
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG645460.12525336755960292No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG48600.024.3523716
TCGACGA53950.022.1453537
CGGTATA76300.020.3174720
GTTCGAC58400.019.9576175
CGACGAG60800.019.3176028
GACCGCG120200.019.1310235
AGACCGC122300.018.9681074
ACCGCGT122150.018.8440366
CAGACCG129300.018.1155193
GTCCGTT50650.017.8157941
GCCGGCT202500.016.9022461
AACGACG32100.016.68203216
CCGCGTT138700.016.6112797
CCGGCTT230050.016.5687962
CGCGTTC142450.016.1584428
CCCGTAT44050.015.9385541
CGTTACG13500.015.66639221
TTCACGC230000.015.6422857
AGTTCGA77900.015.5394854
TCACGCT229800.015.4798998
Produced by FastQC (version 0.11.5)