FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200774

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200774
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48949887
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT652990.1333996950799907No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC153400.021.2414464
CAGACCG161350.020.4179883
CGTTACG20300.019.84013421
GACCGCG164400.019.6011735
ACCGCGT168800.019.1140146
CCGCGTT174750.018.4889567
CGCGTTC176650.018.32818
GAACGAT178300.018.27185445
TTCGACG18950.016.2662146
TCAGACC211050.016.1236762
AATACGG211350.015.39343132
CTCAGAC246000.015.3456641
ATACGGA212100.015.33884233
TACGGAG210450.015.28800334
AGAACGA215350.015.24318944
GCGTTCT227600.014.2944149
AAGAACG235850.013.97552343
GTCCGTT35300.013.7104431
CAATACG242000.013.70413131
ACGGAGA237250.013.6369235
Produced by FastQC (version 0.11.5)