FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200778

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200778
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45861774
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1682920.3669548413020395No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG525760.11464013581332462No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA473930.1033387849323055No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT369000.021.83057245
AGACCGC396650.021.521834
GACCGCG398700.021.3716775
ACCGCGT399500.021.3626636
CAGACCG402300.021.2978523
CCGCGTT414450.020.6083557
CGCGTTC415300.020.5553428
TCAGACC447750.019.4122732
AGAACGA426150.018.88708944
GCGTTCT466300.018.3843569
CTCAGAC501550.018.1802141
ACTCCCC490850.016.71108625
AAGAACG484300.016.62851143
AATACGG485600.016.55223732
CGTTCTC527150.016.52677710
TACGGAG490650.016.36349734
ATACGGA494750.016.2277533
CCCAATA502750.016.15324629
TCGGCGC72300.016.0856845
CCGTACG71950.016.0071494
Produced by FastQC (version 0.11.5)