FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200779

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200779
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45861774
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG855400.18651698907242448No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA492210.10732467522952777No Hit
CCGGGAGGTCACTCTCCCCGGGCTCTGTCCAAGTGGCGTAGGGGAGCATAG472020.10292231608833972No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG262750.016.49179845
GGGAGGT428550.016.2219473
GGAGGTC441900.015.5335384
GGTCACT451550.014.9637017
GTCACTC449200.014.9418218
GGCATAG294900.014.84925135
ATAGGGG297250.014.81049838
TGGCATA310600.014.19916134
GAGCTTA308350.014.11132844
TGGGAGG325650.014.0242792
GAGGTCA505850.013.9392485
TCACTCT490600.013.9194229
ACTCTCC508100.013.13886811
AGGTCAC541750.012.7670826
ACCCGGG129150.012.3854222
CTGGGAG395650.012.37264251
CACTCTC563350.011.9341610
AGCATAG197350.011.7370345
CACCCGG139550.011.7197771
CATAGGG382050.011.63961937
Produced by FastQC (version 0.11.5)