FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200780

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200780
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49895824
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1581820.317024526942375No Hit
CTTGAGATTATCATCCGCTGAGGGTGGAAGCGGCCCCCGCAGACGCTCGGC628220.12590632835325055No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT301500.025.1122545
ACCGCGT319650.024.2449426
GACCGCG321950.023.9811615
AGACCGC325700.023.8305844
CCGCGTT329350.023.5233157
CGCGTTC330900.023.4198368
CAGACCG338650.023.0522773
AGAACGA356050.021.30904844
TCAGACC375200.021.154122
GCGTTCT377050.020.6820289
CTCAGAC428200.019.6791231
TTCGGCG71450.019.55585744
AATACGG390550.019.4661432
TCGGCGC73200.019.08835245
ATACGGA399700.019.01973733
TACGGAG399250.018.90591234
AAGAACG404950.018.77471543
GTTCCTA108500.018.22661815
CCGTACG78650.018.1977484
CGTACGC78750.018.1165965
Produced by FastQC (version 0.11.5)