FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200782

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200782
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44186219
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT594040.13444010676722531No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG466430.10556006161106476No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC132700.021.9407754
CAGACCG140050.020.8696253
GACCGCG150600.019.332945
ACCGCGT159200.018.3150986
TTCGACG13450.017.7309066
GAACGAT165550.017.31354945
CCGCGTT171550.016.9834697
CGCGTTC175200.016.6809568
TCAGACC188350.015.9541932
CTCAGAC215000.015.4448551
GCGTTCT193950.015.1495339
TACGGAG196000.014.73890234
CCGGCTT205100.014.6841782
ATACGGA199150.014.5849733
CCCGTAT28200.014.4693491
AGAACGA198700.014.45902244
AATACGG200750.014.37914932
GTCCGTT35700.014.2080291
CAATACG207900.013.98221531
TTCACGC193700.013.8450327
Produced by FastQC (version 0.11.5)