FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200783

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200783
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44186219
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA464880.10520927350674654No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG21000.013.70953826
GGGAGGT233650.013.5333983
AGCTTAG166700.012.29467245
GGAGGTC257800.012.0646174
ATAGGGG169650.012.0142538
GGCATAG173650.011.62010235
GGTCACT269300.011.1984547
GTCACTC271100.011.0659328
GAGCTTA190300.010.79414744
TGGCATA196900.010.327970534
TCACTCT307900.010.0358819
TGTCGTT29800.09.96280128
TGGGAGG226150.09.9020872
AGGGATC130200.09.7319012
ATATAGC35600.09.666866
ACTCTCC311650.09.61226811
GAGGTCA336350.09.5949715
CACCGAA244200.09.54439245
TAATCGG30100.09.4956714
CATAGGG222050.09.36090737
Produced by FastQC (version 0.11.5)