FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200784

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200784
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47857103
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1010360.21112017582844497No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC218850.023.7605114
GACCGCG224500.022.9420325
CAGACCG227300.022.9265083
ACCGCGT232950.022.1774026
CCGCGTT242150.021.3254367
CGCGTTC244900.021.1113288
GAACGAT264400.019.58073645
TCAGACC283650.019.12582
CTCAGAC327050.018.323311
GCGTTCT284100.018.1904779
AGAACGA301900.017.08136644
AATACGG306400.016.71442232
TACGGAG302900.016.6610234
ATACGGA310350.016.42887533
CCGTACG53600.016.0361844
CAATACG331450.015.72281231
AAGAACG332250.015.55489643
CGTTCTC350300.015.47856810
ACGGAGA328800.015.45109135
CGTACGC56550.015.3985825
Produced by FastQC (version 0.11.5)