FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200785

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200785
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47857103
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG77650.011.00851838
GTTGTCG21400.010.61821526
AGGGATC148800.010.3761622
TCAGACG81250.010.193892
GGCATAG85950.09.97171735
CGTAACG12950.09.72823542
ATAATCG49900.09.020773
CAGGGAT199650.08.9091881
ATCGGTT49900.08.8379156
GGGAGGT173350.08.828753
AGCTTAG97800.08.78778345
GTAACGA41250.08.72818418
TAATCGG51300.08.6398984
TAGCGTA5504.9294613E-108.58905137
CGTGGCG104850.08.3318921
AATCGGT51900.08.1937015
GGGATCA188200.08.1321133
GTCGCGA16900.08.12288915
GGAGGTC186850.07.9953014
GAGCTTA108650.07.95122544
Produced by FastQC (version 0.11.5)