FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200788

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200788
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47333410
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT533960.11280826798660819No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC123950.021.74084
CAGACCG130050.020.963333
GACCGCG138950.019.345265
GAACGAT142150.019.23149945
ACCGCGT149750.018.009596
CCGTACG50600.017.381674
CCGCGTT155200.017.3769267
CGCGTTC158700.017.0787328
TACGGAG163500.016.73296234
ATACGGA164250.016.68352933
CGTACGC54200.016.5591815
AGAACGA167500.016.42858144
AATACGG168050.016.41263232
AACGCTA94850.015.983376523
ACGCTAC96800.015.63816124
AAGAACG179750.015.31413443
CAATACG180350.015.25492231
GCGTTCT180700.015.0243149
CGCTACC102100.014.87044625
CCCGTAT34300.014.7636311
Produced by FastQC (version 0.11.5)