FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200794

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200794
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48146779
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1124570.23357118032755628No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT227550.024.06914745
AGACCGC265850.022.0857244
CAGACCG272600.021.5718353
AGAACGA269400.020.3301244
GACCGCG294850.019.9058515
ACCGCGT302900.019.5018256
CCGCGTT311350.018.9653247
CGCGTTC316500.018.6354038
CCGTACG69350.018.4250934
GCGTTCT324550.018.117739
CGTACGC71300.017.889635
TTCGGCG72650.017.8550444
AAGAACG325850.017.7449243
TCAGACC341650.017.7323882
CTCAGAC365700.017.6338631
TACGGAG333350.017.26761434
ATACGGA334900.017.2140933
AATACGG335650.017.1617932
CAATACG347850.016.61711731
ACGGAGA356750.016.1226835
Produced by FastQC (version 0.11.5)