FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200797

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200797
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44663856
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG590040.13210682033364965No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA544620.12193752371044721No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG450030.10075932539277398No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG158950.012.95149345
GGCATAG160850.012.88286335
GTTGTCG20900.012.81132326
ATAGGGG173900.012.43353938
GGGAGGT271150.012.2406883
GAGCTTA176450.011.81999744
GGAGGTC259500.011.6108554
TGGCATA181450.011.5192834
GGTCACT262350.011.157727
CATAGGG187350.011.09659337
ACTCTCC269600.010.99943411
ACCCGGC65200.010.8713395
GTCACTC274150.010.784168
GAGGTCA283200.010.6550525
AGGGATC148750.010.4379822
GCATAGG202450.010.369019536
CACTCTC290950.010.0454310
AAGCCGG315300.09.52613145
TCACTCT320550.09.4337159
TAGGGGA317300.09.132956539
Produced by FastQC (version 0.11.5)