FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200798

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200798
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41791812
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT651370.15586067433496303No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT136250.023.5963346
AGACCGC138300.023.3935664
GACCGCG138800.023.1304115
CAGACCG144450.022.4755123
CCGCGTT145450.022.1026337
CGCGTTC150350.021.3673298
TCAGACC182100.018.2118512
GCGTTCT189500.017.0953189
GAACGAT189550.016.75854745
CTACACG75000.015.71793645
CTCAGAC245750.015.4271771
AATACGG208200.015.26878832
TACGGAG208650.015.23585734
AGAACGA208400.015.21033444
ATACGGA210350.015.16620333
CCCGTAT28450.014.8330351
GTCCGTT27850.014.6664191
CAATACG226450.014.12785131
CCGTACG62350.014.000874
ACGGAGA228750.013.97564835
Produced by FastQC (version 0.11.5)