FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200799

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200799
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41791812
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT218850.012.6256623
AGCTTAG130900.012.61582345
GGCATAG130450.012.556025535
ATAGGGG135150.012.31964738
GGAGGTC227500.011.8482834
GAGCTTA153450.010.84981944
TGGCATA152850.010.81910934
ACTCTCC252750.010.43206111
GTCACTC256550.010.2514698
GTTGTCG17100.09.73728726
AGGGATC124100.09.7223332
CACTCTC273000.09.61701710
TCAGACG77000.09.4425512
CATAGGG174950.09.4269837
GCATAGG180050.09.19740236
GGCGTAG100300.09.19703535
GCGTAGG100700.09.16083136
CGTAGGG100050.09.13043637
TCACTCT303350.09.0555599
GAGGTCA304950.09.0083695
Produced by FastQC (version 0.11.5)