FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200801

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200801
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46566057
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG95750.012.9483745
GTTGTCG24050.011.69434126
ACCCGGC92800.011.6621435
GGCATAG108800.011.31200835
TAGCGTA14350.011.28917937
GAGCTTA116150.010.86790844
ATAGGGG118350.010.70341538
TCACCCG110500.010.628933
CATAGGG115750.010.59393437
GCGTCGT22800.010.55915811
ATAGCTC96900.010.425739
ACACGGA101900.010.20112616
ACGGACA106850.010.02334718
CACCCGG113150.010.0021814
GCATAGG124150.09.840936
TGGCATA127200.09.74643534
CCGGACA117850.09.54598512
ATAGCGT17250.09.52172236
CACGGAC112950.09.46210417
GACACGG111550.09.35898515
Produced by FastQC (version 0.11.5)