FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005200802

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005200802
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54051366
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1775240.32843573278055543No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACG1037560.1919581458866368No Hit
CCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGA631310.1167981582556119No Hit
CCTGCATGTAAGAATTAAGACCAAGGGAGGGGAGAGAGAAACCCACACATA621380.11496101689640924No Hit
CCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGG579480.10720913140289552No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG32400.022.2863376
GCCGGCT446750.020.9625471
CCGGCTT541800.020.2608972
CCCGCGT15550.019.5878071
CGTTACG30350.019.27047721
TTCACGC580450.018.8188257
TCACGCT592750.018.3751938
CGGCTTC623250.018.0858753
CCCGGCT89650.017.9765911
TCGACGA41700.017.5857227
ACGCGTC43900.017.06330540
ACGCTCA662700.016.68342410
GTTCGAC43150.016.6819445
GTCCGCT39300.016.5997181
CACGCGT46050.016.51099839
CTTCACG686050.016.22386
TCGTTAC24350.015.70461944
CCCGGAT88200.015.6176411
CCCCGCC86800.015.4767291
AGACCGC179250.015.4606944
Produced by FastQC (version 0.11.5)